Molecular identification of blaSHV, blaLEN and blaOKP β-lactamase genes in Klebsiella pneumoniae by bi-directional sequencing of universal SP6- and T7-sequence-tagged blaSHV-PCR amplicons
نویسندگان
چکیده
منابع مشابه
OKP β-lactamase genes in Klebsiella pneumoniae by bi-directional sequencing of universal SP6- and T7-sequence-tagged
Plasmid encoded SHV enzymes represent an important sub-group of class A β-lactamases causing an ESBL phenotype which is increasingly found in Enterobacteriaceae including Klebsiella pneumoniae. The chromosomally encoded β-lactamase LEN and OKP enzymes, which so far only have been found in K. pneumoniae, do not hydrolyse extendedspectrum cephalosporins. In the present study, multiple displacemen...
متن کاملMolecular identification of CTX-M and blaOXY/K1 β-lactamase genes in Enterobacteriaceae by sequencing of universal M13-sequence tagged PCR-amplicons
BACKGROUND Plasmid encoded blaCTX-M enzymes represent an important sub-group of class A beta-lactamases causing the ESBL phenotype which is increasingly found in Enterobacteriaceae including Klebsiella spp. Molecular typing of clinical ESBL-isolates has become more and more important for prevention of the dissemination of ESBL-producers among nosocomial environment. METHODS Multiple displacem...
متن کاملPrevalence and Molecular Characterization of Plasmid-mediated Extended-Spectrum β-Lactamase Genes (balaTEM, blaCTX and blASHV) Among Urinary Escherichia coli Clinical Isolates in Mashhad, Iran
Objective(s) Extended-spectrum beta-lactamase (ESBL) producing bacteria have an important role in nosocomial infections. Due to the limited availability of information about the molecular epidemiology of ESBL producing bacteria in Mashhad, we decided to investigate about TEM, CTX and SHV ESBLs among urinary Escherichia coli isolates in Mashhad, a city in northeast Iran. Materials and Methods ...
متن کاملPlasmid-borne blaSHV genes in Klebsiella pneumoniae are associated with strong promoters.
BACKGROUND Extended-spectrum beta-lactamases (ESBLs) belonging to the SHV family remain a major cause of ESBL-positive phenotypes in Klebsiella pneumoniae. The bla(SHV) gene is a normal constituent of the K. pneumoniae chromosome. However, most ESBL-encoding bla(SHV) genes found in K. pneumoniae are plasmid borne. The objective was to determine the contribution of promoter variants to the expre...
متن کاملPrevalence of Extended-spectrum Beta-lactamases (ESBL) Types blaTEM and blaSHV in Klebsiella pneumoniae Strains Isolated from Clinical Samples by PCR in Miandoab, West Azerbaijan
Background and Objective: Beta-lactamases are the most important factors in the resistance to beta-lactam antibiotics among gram-negative bacteria, especially Klebsiella pneumoniae. Nowadays, the prevalence of infections caused by extended-spectrum β-lactamases (ESBLs)-producing K. pneumoniae is increasing, as one of the emerging health problems throughout the world. This study aimed to investi...
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ژورنال
عنوان ژورنال: Molecular and Cellular Probes
سال: 2009
ISSN: 0890-8508
DOI: 10.1016/j.mcp.2009.01.005